目的 建立淡豆豉药材中4种黄酮类成分同时测定的一测多评含量测定方法。方法 色谱条件:采用Diamonsil-C₁₈色谱柱(4.6 mm×250 mm,5 μm),流动相甲醇(A)-0.5%冰醋酸溶液(B),梯度洗脱(0~25 min,30%~45%A,25~50 min,45%~60%A),流速1.0 mL·min^-1,柱温30 ℃,检测波长260 nm。以大豆苷元为指标,建立药材中该成分与大豆苷、染料木苷及染料木素的相对校正因子,利用校正因子计算4种黄酮类成分的含量,实现一测多评,同时采用外标法测定4种黄酮类成分的含量,比较计算值与实测值的差异。结果 15批淡豆豉药材中4种黄酮类成分的含量可以用一测多评法进行测定,建立的校正因子重复性良好,采用校正因子计算的含量值与外标法实测值之间无显著差异。结论 以外标法测定大豆苷元,利用相对校正因子实现大豆苷,染料木苷,染料木素测定是准确的,可行的,一测多评法可以用于特定的不同类型成分间的测定。

OBJECTIVE To develop a method of quantitative analysis of multi-components by single marker (QAMS) for simultaneous determination of daidzein, daidzin, genistein and genistin in Semen Sojae Praeparatum. METHODS Chromatographic conditions were:Diamonsil-C₁₈ column (4.6 mm×250 mm,5 μm), mobile phase of methanol(A)-0.5% acetic acid(B),gradient elution (0-25 min,30%-45%A,25-50 min,45%-60%A),flow rate 1.0 mL·min^-1,column temperature 30 ℃,detection wavelength 260 nm. An HPLC method was developed as QAMS to determine the four components of soybean isoflavones in Semen Sojae Praeparatum. Daidzein was selected as the internal reference substance, and the relative correlation factors (RCF) of daidzin, genistein and genistin were determined by HPLC with good reproducibility. The contents of the four components in 15 batches of sample were determined by QAMS and external standard method. RESULTS No significant differences were observed between the quantitative analysis RESULTS of QAMS method and external standard method in 15 batches of Semen Sojae Praeparatum. CONCULSION It is feasible and suitable to evaluate the quality of Semen Sojae Praeparatum by QAMS.